Biolegend, 346707, APC anti-human NKp80 Antibody, 25tests

NKp80, also known as killer cell lectin-like receptor subfamily F, member 1 (KLRF1), is a type II transmembrane C-type lectin-like receptor. NKp80 is expressed on the cell surface of nearly all NK cells as a homodimer with approximately 80 kD molecular mass. It is also found on a subset of effector memory CD8 T cells and γ/δ T cells. NKp80 induces NK-cell mediated cytotoxicity and cytokine production through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant ectodomain of human NKp80
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Welte S, et al. 2006. Nat. Immunol. 7:1334. Kuttruff S, et al. 2009. Blood 113:358.
Product Citations: Orta-Resendiz A, et al. 2023. STAR Protoc. 102734:4. PubMed Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed
RRID: AB_2044041 (BioLegend Cat. No. 346708)
Structure: Type II transmembrane c-type lectin-like receptor, homodimer, 80 kD
Distribution: NK cells, subset of CD8 T and γ/δ T cells
Ligand/Receptor: AICL (activation-induced C-type letcin)
Bioactivity: induce NK cell-mediated cytolysis and cytokine release
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Antigen References: 1. Kuttruff S, et al. 2009. Blood 113:358 2. Vitale M, et al. 2001. Eur. J. Immunol. 31:233
Gene ID: 51348
UniProt: View information about NKp80 on UniProt.org
Clone: 5D12
Regulatory Status: RUO
Other Names: KLRF1, killer cell lectin-like receptor subfamily F, member 1
Isotype: Mouse IgG1, κ