Biolegend, 347603, PE anti-human Siglec-10 Antibody, 25tests

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-10 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Munday J, et al. 2001. Biochem. J. 355:489 Yokoi H, et al. 2006. Allergy. 61:769 Kivi E, et al. 2009. Blood 114:5385 Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
Product Citations: Büll C, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.105. PubMed Wang Z, et al. 2020. Cell Mol Immunol. 17:1148. PubMed
RRID: AB_2270417 (BioLegend Cat. No. 347603) AB_2189155 (BioLegend Cat. No. 347604)
Structure: Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution: Monocytes, eosinophils, B cells, and a subset of NK cells
Function: Cell adhesion, signal transduction
Interaction: SHP-1, PTPN6
Ligand/Receptor: CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity: Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type: B cells, Eosinophils, Monocytes, NK cells
Biology Area: Immunology
Molecular Family: Siglec Molecules
Antigen References: 1. Chen GY, et al. 2009. Science 323:1722 2. Aizawa H, et al. 2003. Genomics.82:521 3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355
Gene ID: 89790
UniProt: View information about Siglec-10 on UniProt.org
Clone: 5G6
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 10
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.