Biolegend, 347619, Brilliant Violet 421™ anti-human Siglec-10 Antibody, 25tests

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-10 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Munday J, et al. 2001. Biochem. J. 355:489 Yokoi H, et al. 2006. Allergy. 61:769 Kivi E, et al. 2009. Blood 114:5385 Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
RRID: AB_3083335 (BioLegend Cat. No. 347619) AB_3083335 (BioLegend Cat. No. 347620)
Structure: Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution: Monocytes, eosinophils, B cells, and a subset of NK cells
Function: Cell adhesion, signal transduction
Interaction: SHP-1, PTPN6
Ligand/Receptor: CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity: Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type: B cells, Eosinophils, Monocytes, NK cells
Biology Area: Immunology
Molecular Family: Siglec Molecules
Antigen References: 1. Chen GY, et al. 2009. Science 323:1722 2. Aizawa H, et al. 2003. Genomics.82:521 3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355
Gene ID: 89790
UniProt: View information about Siglec-10 on UniProt.org
Clone: 5G6
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 10
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.