Biolegend, 348008, Purified anti-human Granulysin Antibody, 500μg

Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant 9 kD granulysin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 4 µg/ml of the capture antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
Application Notes: Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1 ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard. Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations. Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Hanson DA, et al. 1999. Mol. Immunol. 36:413. Spada FM, et al. 2000. J. Exp. Med. 191:937.
Product Citations: Peeters MJW, et al. 2021. Front Immunol. 12:718863. PubMed
RRID: AB_2563601 (BioLegend Cat. No. 348007) AB_2563602 (BioLegend Cat. No. 348008)
Structure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution: NK cells, cytolytic T cells
Function: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type: NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Krensky AM. 2000. Biochem. Pharmacol. 59:317. 2. Deng A, et al. 2005. J. Immunol. 174:5243. 3. Kaspar AA, et al. 2001. J. Immunol. 167:350. 4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.
Gene ID: 10578
UniProt: View information about Granulysin on UniProt.org
Clone: DH2
Regulatory Status: RUO
Other Names: GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype: Mouse IgG1, κ