Biolegend, 349133, Spark UV™ 387 anti-human CD235a (Glycophorin A) Antibody, 25tests

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm.
Excitation Laser: Ultraviolet Laser (355 nm)
Application References(PubMed link indicates BioLegend citation): Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
RRID: AB_3097367 (BioLegend Cat. No. 349133) AB_3097367 (BioLegend Cat. No. 349134)
Structure: Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution: Erythrocytes and erythroid precursors
Function: Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor: Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type: Erythrocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Reid ME. 2009. Immunohematology 25:95. 2. Palacajornsuk P. 2006. Immunohematology 22:171. 3. Pasvol G. 2003. Trends Parasitol. 19:430. 4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.
Gene ID: 2993
UniProt: View information about CD235a on UniProt.org
Clone: HI264
Regulatory Status: RUO
Workshop: VII 70312
Other Names: PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, GYPA
Isotype: Mouse IgG2a, κ