Biolegend, 349754, PE anti-human CD303 (BDCA-2) Antibody, 100tests

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CLEC4C-transfectants (full length)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W19421D shows minimal or no non-specific binding to the monocyte compared to both the current catalogue clones (201A & S19008G). Clone W19421D does not cross block with clone 201A. Clone W19421D cross blocks with clone S19008G.
RRID: AB_3720483 (BioLegend Cat. No. 349753) AB_3720483 (BioLegend Cat. No. 349754)
Structure: Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution: Plasmacytoid dendritic cells
Function: Antigen capture, inhibition of IFN α/β production
Cell Type: Dendritic cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Jähn PS, et al. 2010. Cell Immunol. 265:15. Graham LM and Brown GD. 2009. Cytokine. 48:148. Riboldi E, et al. 2011. J Biol Chem. 2011; 286:35329-33. Shinde R, et al. 2018. Nat Immunol. 2018; 19:571-582.
Gene ID: 170482
UniProt: View information about CD303 on UniProt.org
Clone: W19421D
Regulatory Status: RUO
Other Names: CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.