Biolegend, 350024, Biotin anti-human CD134 (OX40) Antibody, 100μg

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4 + T cell tolerance.
100μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV 1-transformed HUT 102 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP) Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC) Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC) Willett B, et al. 2007. J. Virol. 81:9665. (ELISA) Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA) Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
RRID: AB_2571943 (BioLegend Cat. No. 350024)
Structure: 50 kD, TNF receptor family
Distribution: Activated T cells
Function: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor: OX40L (CD252)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Smith CA, et al. 1994. Cell. 76:959. 2. Chen AL, et al. 1999. Immunity. 11:689. 3. Croft M. 2010. Annu. Rev. Immunol. 28:57. 4. Withers DR, et al. 2009. J. Immunol. 183:5079. 5. Klinger M, et al. 2009. J. Immunol. 182:4581.
Gene ID: 7293
UniProt: View information about CD134 on UniProt.org
Clone: Ber-ACT35 (ACT35)
Regulatory Status: RUO
Other Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.