Biolegend, 350104, Alexa Fluor® 647 anti-human CD294 (CRTH2) Antibody, 100tests

CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Rat cell line TART/B19-12.10 transfected with human CRTH2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This product is sold under license agreement with BML, Inc for research use only.
Application References(PubMed link indicates BioLegend citation): Nagata K, et al. 1999. J. Immunol. 162:1278. (FC) De Fanis U, et al. 2007. Blood 109:4343. (FC) Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC) Hamada K, et al. 2004. Allergology International 53:179. (FC)
Product Citations: Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed Witkowski M, et al. 2021. Nature. 600:295. PubMed Wright AKA, et al. 2017. J Allergy Clin Immunol. 140:1430. PubMed Vijayakumar B, et al. 2022. Immunity. . PubMed Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
RRID: AB_10640066 (BioLegend Cat. No. 350103) AB_10642025 (BioLegend Cat. No. 350104)
Structure: Seven-transmembrane protein coupled with G proteins
Distribution: Effector T helper 2 (Th2) cells, eosinophils, basophils
Function: Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Interaction: Heterotrimeric G proteins
Ligand/Receptor: Prostaglandin D2
Cell Type: Basophils, Eosinophils, Th2
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711. 2. Monneret G, et al. 2001. Blood 98:1942. 3. Xue L, et al. 2009. J. Immunol. 182:7580. 4. Schratl P, et al. 2007. J. Immunol. 179:4792.
Gene ID: 11251
UniProt: View information about CD294 on UniProt.org
Clone: BM16
Regulatory Status: RUO
Other Names: Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
Isotype: Rat IgG2a, κ