Biolegend, 350312, PerCP/Cyanine5.5 anti-human CD1d Antibody, 100tests

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to i NKT cells analogous to MHC molecule presentation of peptides to T cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD1d-Fc fusion
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: "Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. "
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
Application References(PubMed link indicates BioLegend citation): Exley M, et al. 2000. Immunology 100:37. (IHC, WB) Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB) Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
Product Citations: Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed
RRID: AB_2562409 (BioLegend Cat. No. 350311) AB_2562410 (BioLegend Cat. No. 350312)
Structure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function: Present lipid antigens to iNKT cells
Ligand/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819. 2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010. 3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889. 4. Teige A, et al. 2010. J. Immunol. 185:345.
Gene ID: 912
UniProt: View information about CD1d on UniProt.org
Clone: 51.1
Regulatory Status: RUO
Other Names: R3G1
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.