Biolegend, 350314, Brilliant Violet 510™ anti-human CD1d Antibody, 100tests

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to i NKT cells analogous to MHC molecule presentation of peptides to T cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD1d-Fc fusion
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
Application References(PubMed link indicates BioLegend citation): Exley M, et al. 2000. Immunology 100:37. (IHC, WB) Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB) Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
Product Citations: Munteanu AN, et al. 2019. Exp Ther Med. 18:1693. PubMed
RRID: AB_2563190 (BioLegend Cat. No. 350314)
Structure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function: Present lipid antigens to iNKT cells
Ligand/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819. 2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010. 3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889. 4. Teige A, et al. 2010. J. Immunol. 185:345.
Gene ID: 912
UniProt: View information about CD1d on UniProt.org
Clone: 51.1
Regulatory Status: RUO
Other Names: R3G1
Isotype: Mouse IgG2b, κ