Biolegend, 351403, PE anti-human CD65 Antibody, 25tests

CD65, also referred to as ceramide-dodecasaccharide or type II fucoganglioside, is a fucosylated carbohydrate antigen which serves as a ligand for CD62E, commonly known as E-selectin. A key difference between CD65 and CD65s is that CD65 does not have a terminal sialic acid, although the rest of their structures are identical. CD65 is expressed on most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood, and plays a role in cell adhesion. It has been identified as a key adhesive ligand that plays a significant role in the extravascular infiltration of cells associated with acute myeloid leukemia (AML).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: THP-1 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Lund­-Johansen F, et al. 1992. J Immunol. 148:3221-9. Bordessoule D, et al. 1993. Br J Haematol. 83:370-83. Kniep B, et al. 1996. J. Biochem 119:456-62.
RRID: AB_3683373 (BioLegend Cat. No. 351403) AB_3683373 (BioLegend Cat. No. 351404)
Structure: The structure of CD65 is defined as Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide.
Distribution: Most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood.
Function: Cell adhesion
Ligand/Receptor: Serve as a ligand for CD62E, also known as E-selection
Cell Type: Granulocytes, Leukocytes, Monocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: Joziasse DH, et al. 1989. J. Biol. Chem. 264:14290-7.
Gene ID: NA
Clone: VIM8
Regulatory Status: RUO
Other Names: Ceramide dodecasacharide, VIM2
Isotype: Mouse IgM, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.