Biolegend, 351601, Purified anti-human Integrin α9β1 Antibody, 25μg

Integrin α9β1 is a transmembrane protein belonging to the integrin family. It is widely expressed in airway epithelium, different types of muscle cells, and hepatocytes. In the hematopoietic system, integrin α9β1 is highly expressed on human neutrophils and at low level on monocytes. Multiple ligands have been identified for this integrin including the inducible endothelial counter-receptor, vascular cell adhesion molecule-1 (VCAM-1), the extracellular matrix proteins tenascin C and osteopontin, some members of ADAMs family, coagulation factor XIII, and von Willebrand factor. It has been reported that binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9β1 enhancement of cell migration, mediates adhesion to the extracellular matrix proteins, and is involved in G-CSF signaling. More recently, integrin α9β1 was found on hematopoietic stem and progenitor cells to mediate stem cells adhesion, proliferation, and differentiation.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: L-cells transfected with human α9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported application (for the relevant formats) include: immunofluorescence microscopy1, immunopricipitation2, and blocking3.
Application References(PubMed link indicates BioLegend citation): Lafuste P, et al. 2005. Mol. Biol. Cell. 16:861. (IF) Chen C, et al. 2006. Immunity 25:895. (FC, IP) Taooka Y, et al. 1999. J. Cell Biol. 145:413. (Block)
Product Citations: Mashinchian O, et al. 2022. EMBO J. 41:e110655. PubMed
RRID: AB_10901165 (BioLegend Cat. No. 351602)
Disclaimer: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Structure: Heterodimeric proteins made up of α9 and β1 subunits
Distribution: Neutrophil, monocytes, endothelial cells, hepatopoietic cells, and different type of muscle cells
Function: Receptor for VCAM-1, involved in cell adhesion and migration
Interaction: Recognizes α9 integrin expressed as a heterodimer in association with the β1 integrin.
Ligand/Receptor: VCAM-1, ECM proteins tenascin C, osteopontin
Cell Targets: Binds VCAM-1
Cell Type: Endothelial cells, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Schreiber TD, et al. 2009 Haematologica 94:1495. 2. Chen C, et al. 2006 Immunity 25:895. 3. Taooka Y, et al. 1999 J. Cell Biol. 145:413. 4. Palmer EL, et al. 1993 J. Cell Biol. 123:1289.
Gene ID: 36803688
UniProt: View information about Integrin alpha9beta1 on UniProt.org
Clone: Y9A2
Regulatory Status: RUO
Other Names: ITGA9, ITGB1
Isotype: Mouse IgG1, κ