Biolegend, 352501, Purified anti-human GARP (LRRC32) Antibody, 25μg

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LRRC32-DNA vaccination
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_10897447 (BioLegend Cat. No. 352502)
Structure: 80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution: Activated Tregs, megakaryocytes, platelets
Ligand/Receptor: LAP (TGF-β1)
Cell Type: Megakaryocytes, Platelets, Tregs
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213. 2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315. 3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439. 4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
Gene ID: 2615
UniProt: View information about GARP on UniProt.org
Clone: 7B11
Regulatory Status: RUO
Other Names: Garpin, Glycoprotein A repetitions predominant
Isotype: Mouse IgG2b, κ