Biolegend, 352603, PE anti-human CD220 Antibody, 25tests

CD220, also known as insulin receptor, is a type I transmembrane receptor tyrosine kinase composed of two extracellular α-subunits and two transmembrane β-subunits. Binding insulin, the insulin receptor forms a heterotetramer of two units to induce autophosphorylation and activation of the tyrosine kinase activity of the receptor.  Activation of insulin receptor leads to subsequent downstream signaling in metabolic regulation, inducing glucose uptake, cell growth, differentiation, and apoptosis. Gene mutation in the insulin receptor or decreased insulin receptor signaling leads to insulin-resistant diabetes mellitus and noninsulin-dependent diabetes mellitus (diabetes mellitus type 2). Most normal cells constitutively express insulin receptors. In hematopoietic cells, insulin receptor is constitutively expressed on monocytes and selectively expressed on activated lymphocytes.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human insulin receptor/freund's adjuvant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lozza L, et al. 2014. Eur J Immunol. 80:44. PubMed Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed Kim H, et al. 2012. PLoS One. e:47454. PubMed
RRID: AB_10898328 (BioLegend Cat. No. 352603) AB_10933245 (BioLegend Cat. No. 352604)
Structure: Consist of two alpha subunits and two beta subunits; the beta subunit passes through the cellular membrane and is linked by disulfide bonds.
Distribution: Hematopoietic and non hematopoietic cells.
Function: Regulates cell metabolism and growth.
Interaction: Ectonucleotide pyrophosphatase/phosphodiesterase, PTPN11, GRB10, GRB7, PRKCD, IRS1, SH2B1 and Mothers against decapentaplegic homolog 2.
Ligand/Receptor: Insulin
Biology Area: Cell Biology, Immunology, Protein Synthesis, Signal Transduction
Molecular Family: CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Viardot A, et al. 2006. Endocrinology 148:346. 2. Ward CW, et al. 2009. Bioessays 31:422. 3. Brindle NP, et al. 1990. Biochem. J. 268:615.
Gene ID: 3643
UniProt: View information about CD220 on UniProt.org
Clone: B6.220
Regulatory Status: RUO
Other Names: Insulin Receptor
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.