Biolegend, 353206, Alexa Fluor® 488 anti-human CD197 (CCR7) Antibody, 100tests

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed Braun J, et al. 2020. Nature. 587:270. PubMed Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed Lerrer S, et al. 2021. iScience. 24:103020. PubMed Balan I, et al. 2022. Front Immunol. 13:940095. PubMed Woodruff M, et al. 2020. medRxiv. . PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed Serrano I, et al. 2022. Front Immunol. 13:883743. PubMed Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed Ferreira-Gomes M, et al. 2021. Nat Commun. 12:1961. PubMed
RRID: AB_10918624 (BioLegend Cat. No. 353205) AB_10916389 (BioLegend Cat. No. 353206)
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236
UniProt: View information about CD197 on UniProt.org
Clone: G043H7
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.