Biolegend, 353303, PE anti-human Perforin Antibody, 25tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Kim S, et al. 2023. Front Immunol. 14:1062365. PubMed Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed Figueroa-Romero C, et al. 2022. Front Immunol. 13:773288. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed Buggert M, et al. 2014. J Immunol. 192:4685. PubMed Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed Brumeanu TD, et al. 2020. bioRxiv. . PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed Collins DR, et al. 2021. Immunity. 54:2372. PubMed Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed Patel T, et al. 2017. Cytotechnology. 10.1007/s10616-017-0106-3. PubMed Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID: AB_10915476 (BioLegend Cat. No. 353303) AB_2616860 (BioLegend Cat. No. 353304)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis.
Cell Type: T cells, NK cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: B-D48
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG1
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.