Biolegend, 353313, PerCP/Cyanine5.5 anti-human Perforin Antibody, 25tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Xing JH, et al. 2024. Microbiome. 20:12. PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed van Montfoort N, et al. 2018. Cell. 175:1744. PubMed Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID: AB_2571970 (BioLegend Cat. No. 353313) AB_2571971 (BioLegend Cat. No. 353314)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis.
Cell Type: NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: B-D48
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG1
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.