Biolegend, 353804, PE anti-human CD370 (CLEC9A/DNGR1) Antibody, 100tests

CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16 - monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Luo X, et al. 2023. Methods Mol Biol. 2618:133. PubMed Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed Lee J, et al. 2015. J Exp Med. 212:385. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Breton G, et al. 2015. J Exp Med. 212:401. PubMed Wimmers F, et al. 2021. Cell. 184:3915. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
RRID: AB_10959651 (BioLegend Cat. No. 353803) AB_10965546 (BioLegend Cat. No. 353804)
Structure: Type II transmembrane glycoprotein; consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM like motif, a 21 aa transmembrane segment, a 185 aa extracellular domain (ECD) that contains a stalk region, and one C-type lectin domain (CTLD).
Distribution: Dendritic subsets including plasmacytoid dendritic cells.
Function: Cross-present antigen to CTL, phagocytosis necrotic cells.
Interaction: Necrotic cells.
Ligand/Receptor: Ubiquitous preformed acid-labile protein associated ligand(s).
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens
Antigen References: 1. Poulin LF, et al. 2010. J. Exp. Med. 207:1261. 2. Sancho D, et al. 2009. Nature 458:899. 3. Huysamen C, et al. 2008. J. Biol. Chem. 283:16693.
Gene ID: 283420
UniProt: View information about CD370 on UniProt.org
Clone: 8F9
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: C-type lectin family member 9A, CD370
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.