Biolegend, 354603, PE anti-human CD290 (TLR10) Antibody, 25tests

CD290, also known as TLR10, is a 91-100 kD glycosylated protein belonging to the toll-like receptor family. It is expressed on B cell, Treg cells, and plasmacytoid dendritic cells but absent on T cells, particularly non-Treg and granulocytes. It can interact with MyD88 and plays an important role in innate immunity and pattern recognition.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified extracellular domain of human TLR10 (amino acids 20-474) (NP_001017388 )
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Guan Y, et al. 2010. J. Immunol. 184:5094.
RRID: AB_11219598 (BioLegend Cat. No. 354603) AB_11219791 (BioLegend Cat. No. 354604)
Structure: Member of the toll-like receptor family, type I membrane protein, 91-100 kD
Distribution: B cells, Treg cells, plasmacytoid DCs
Function: Interacts with MyD88 and TRAF6, which results in NF-kB activation and cytokine secretion
Ligand/Receptor: MyD88 and TRAF6
Cell Type: B cells, Dendritic cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Chuang T, et al. 2001. Biochim. Biophys. Acta. 1518:157. 2. Hornung V, et al. 2002. J. Immunol. 168:4531. 3. Bourke E, et al. 2003. Blood 102:956.
Gene ID: 81793
UniProt: View information about CD290 on UniProt.org
Clone: 3C10C5
Regulatory Status: RUO
Other Names: Toll like receptor 10
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.