Biolegend, 354909, FITC anti-human CD21 Antibody, 25tests

CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.
Application References(PubMed link indicates BioLegend citation): Björck P, et al. 1993. Eur. J. Immunol. 23:1771. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
Product Citations: Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed Jiang C, et al. 2020. Cell Reports. 28(5):1307-1322.e8.. PubMed Nishio A, et al. 2022. Nat Commun. 13:5446. PubMed
RRID: AB_2561574 (BioLegend Cat. No. 354909) AB_2561575 (BioLegend Cat. No. 354910)
Structure: Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
Distribution: Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells
Function: Epstein-Barr virus receptor on B cells used for infection
Interaction: CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
Ligand/Receptor: C3d, EBV
Cell Type: B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area: Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc. 2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194. 3. Szakonyi G, et al. 2001. Science 292:1725. 4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.
Gene ID: 1380
UniProt: View information about CD21 on UniProt.org
Clone: Bu32
Regulatory Status: RUO
Workshop: V CD21.4, VI CD21.5
Other Names: Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Isotype: Mouse IgG1, κ