Biolegend, 355111, PE/Cyanine7 anti-human CD70 Antibody, 25tests

CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. CD70 is expressed on activated T, B and NK cells, activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia and large B cell lymphomas. CD70 costimulates T cell proliferation and differentiation. It plays a role in the pDC-induced B cell differentiation. The ligand of CD70 is CD27.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD70-transfected L cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: blocking of plasmacytoid dendritic cell induced B cell proliferation and Ig secretion1.
Application References(PubMed link indicates BioLegend citation): Shaw J, et al. 2010. Blood 115:3051. (Block)
Product Citations: Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed
RRID: AB_2687253 (BioLegend Cat. No. 355111) AB_2687254 (BioLegend Cat. No. 355112)
Structure: Type II transmembrane glycoprotein, member of the tumor necrosis factor superfamily, 50 kD
Distribution: Activated T cells and B cells, activated NK cells, activated plasmacytoid dendritic cells, some B cell lymphomas
Function: Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation
Ligand/Receptor: CD27
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Bowman MR, et al. 1994. J. Immunol. 152:1756. 2. Shaw J, et al. 2010. Blood 115:3051. 3. Keller AM, et al. 2009. Blood 113:5167.
Gene ID: 970
UniProt: View information about CD70 on UniProt.org
Clone: 113-16
Regulatory Status: RUO
Other Names: CD27 ligand, CD27L, Ki-24 antigen, Tumor necrosis factor ligand superfamily, member 7 (TNFSF7)
Isotype: Mouse IgG1, κ