Biolegend, 355403, PE anti-human CD369 (Dectin-1/CLEC7A) Antibody, 25tests

CD369 (Dectin-1), also known as CLEC7A and beta-glucan receptor (BGR), is a 33 kD type II transmembrane C-type lectin. It consists of a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a short N-terminal ITAM containing a cytoplasmic tail. CLEC7A is predominantly expressed on dendritic cells. Subsequent work has shown expression on B cells, monocytes, eosinophils, mast cells, macrophages, endothelial cells, and low expression on some subsets of T cells. CLEC7A lacks residues involved in calcium ligation that mediates carbohydrate-binding by classical C-type lectins. CLEC7A functions as a receptor to mediate innate immunity to fungal pathogens by selectively binding beta-glucan polymers, a major component of yeast and mycobacterial cell walls.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain with C-term hFc fusion, RIBLS.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The epitope of clone 15E2 is LWEDGSTFSSN. Clone 15E2 can recognize the epitope in the presence of Curdlan and Laminarin (CLEC7A ligands). Additional reported applications (for the relevant formats) include: immunohistochemical staining.1
Application References(PubMed link indicates BioLegend citation): Ni L, et al. 2010. J. Immunol. 185:3504. (IHC)
Product Citations: Zhang H, et al. 2020. Nat Cancer. 1:826. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Barresi V, et al. 2020. J Clin Med. 9:00. PubMed Verma M, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_2562060 (BioLegend Cat. No. 355403) AB_2562061 (BioLegend Cat. No. 355404)
Structure: Type II transmemberane C-type lectin, one carbohydrate recognition domain, ITAM containing cytoplasmic tail
Distribution: B cells, low level of some subsets of T cells, monocytes, dendritic cells, macrophages and endothelial cells
Function: Mediates innate immunity to fungal pathogens
Interaction: TLR2, CD37
Ligand/Receptor: Beta-glucan
Cell Type: B cells, Dendritic cells, Endothelial cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Goodridge HS, et al. 2011. Nature 472:471. 2. Saijo S, et al. 2007. Nat. Immunol. 8:39. 3. Brown GD. 2006. Nat. Rev. Immunol. 6:33. 4. Willment JA, et al. 2001. J. Biol. Chem. 276:43818. 5. Yokota K, et al. 2001. Gene 272:51.
Gene ID: 64581
UniProt: View information about CD369 on UniProt.org
Clone: 15E2
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CLECSF12, beta-glucan receptor (BGR, betaGR), CD369
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.