Biolegend, 357405, FITC anti-human CD4 Antibody, 25tests

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD4 T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Lilliebladh S, et al. 2018. J Immunol Res. 2018:6984563. PubMed Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed Martinez-Fabregas J, et al. 2021. STAR Protocols. 2(2):100417. PubMed Katrin Schaper‐Gerhardt et al. 2018. British journal of pharmacology. 1111/bph. PubMed Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed Padmanabhan U, et al. 2020. Eur J Haematol. 104:170. PubMed Lu Y, et al. 2019. Anticancer Res. 39:5911. PubMed Parenti S, et al. 2021. NPJ Precis Oncol. 5:4. PubMed Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Grant R, et al. 2021. Methods Protoc. 4:. PubMed Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed Wilmes S, et al. 2021. Elife. 10:. PubMed Kelly DL, et al. 2018. Psychiatry Res. 269:517. PubMed Angela M, et al. 2016. Nat Commun. 7:13683. PubMed McGowan J, et al. 2019. Front Immunol. 10:1553. PubMed van Galen P, et al. 2019. Cell. 176:1265. PubMed
RRID: AB_2562356 (BioLegend Cat. No. 357405) AB_2562357 (BioLegend Cat. No. 357406)
Structure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution: Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
Interaction: Part of the TCR/CD3 complex
Ligand/Receptor: β2 domain from the MHC class II
Cell Type: Macrophages, Monocytes, NKT cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: A161A1
Regulatory Status: RUO
Other Names: T4/Leu-3
Isotype: Rat IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.