Biolegend, 358202, Purified anti-human GPR56 Antibody, 100μg

GPR56, also known as TM7XN1, is an orphan G-protein-coupled receptor (GPCR) containing seven transmembrane domains and a mucin-like domain in the N-terminal region. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons and encodes 693 amino acids.  GPR56 is expressed in a wide range of tissues, such as brain, thyroid gland, and heart. Mutations in human GPR56 gene were found to cause a specific brain malformation called bilateral frontoparietal polymicrogyria. Recently, GPR56 was found specifically expressed on cytotoxic NK and T lymphocytes, including CD8+, CD4+, and γδ T cells, which might affect the migratory properties of these cells. GPR56 was also found significantly downregulated in some cancer cells, suggesting that it may play a role in growth suppression and cancer metastasis.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR56-mouse Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone CG4 cross-reacts with mouse1.
Application References(PubMed link indicates BioLegend citation): Rao TN, et al. 2015. Stem Cell Res. 14:307. (FC) PubMed
Product Citations: Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
RRID: AB_2562082 (BioLegend Cat. No. 358202)
Structure: Contains seven transmembrane domains and a mucin-like domain in the N-terminal region
Distribution: T cells, NK cells, wide range of tissues such as brain, thyroid gland, and heart
Function: May be involved in immune cell migration, cancer cell growth metastasis, and neuron development
Cell Type: NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Peng YM, et al. 2011. J. Leukoc. Biol. 90:735. 2. Shashidhar S, et al. 2005. Oncogene 24:1673. 3. Piao X, et al. 2004. Science 303:2033. 4. Liu M, et al. 1999. Genomics 55:296.
Gene ID: 9289
UniProt: View information about GPR56 on UniProt.org
Clone: CG4
Regulatory Status: RUO
Other Names: TM7XN1
Isotype: Mouse IgG1, κ