Biolegend, 358606, APC anti-human LOX-1 Antibody, 100tests

Lectin-like oxidized LDL receptor 1 (LOX-1) also known as CLEC8A is a 31 kD single pass type II transmembrane protein and member of the C-type lectin superfamily. LOX-1 is a scavenger receptor and is involved in endocytosis, phagocytosis and cytokine production. CLEC8A is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands including lipoproteins, apoptotic cells heat shock proteins, C-reactive protein and oxidized LDL. LOX-1 is implicated in atherosclerosis and vascular inflammation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LOX-1 ectodomain: Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1.
Application References(PubMed link indicates BioLegend citation): Li D, et al. 2012. J. Exp. Med. 209:109. (IHC)
Product Citations: Bayik D, et al. 2022. Cancer Res. 82:4274. PubMed Weed DT, et al. 2019. Front Immunol. 10:1206. PubMed Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed Chmielowski R, et al. 2017. Acta Biomaterialia. 10.1016/j.actbio.2017.05.029. PubMed
RRID: AB_2563472 (BioLegend Cat. No. 358605) AB_2563473 (BioLegend Cat. No. 358606)
Structure: Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily.
Distribution: Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli
Function: Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production
Ligand/Receptor: Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL
Cell Type: Endothelial cells, Platelets, Fibroblasts, Macrophages, Dendritic cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: 1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 4. Lin FY, et al. 2011. J. Immunol. 186:4405. 5. Parlato S, et al. 2010. Blood 115:1554.
Gene ID: 4973
UniProt: View information about LOX-1 on UniProt.org
Clone: 15C4
Regulatory Status: RUO
Other Names: OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1
Isotype: Mouse IgG2a, κ