Biolegend, 359505, PE anti-human CD360 (IL-21R) Antibody, 25tests

Human interleukin 21 receptor (IL-21R) is a single pass type I membrane protein and a member of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibits greatest extracellular homology to the IL-2R beta subunit (i.e., it contains one copy of the WSXWS-containing cytokine-binding domain). Intracellular domains of IL-21R include the Box 1 and Box 2 elements, which are similar to the IL-9R intracellular region. Upon binding IL-21, IL-21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Stat signaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is a potent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly the inflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells, and can be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-21R expressing mouse M1-T22 cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescent surface staining1.
Application References(PubMed link indicates BioLegend citation): Bannantine J, et al. 2011. Front Microbiol. 2:163. (IF)
Product Citations: Thomson Z, et al. 2023. Nat Immunol. 1947:24. PubMed
RRID: AB_2562368 (BioLegend Cat. No. 359505) AB_2562369 (BioLegend Cat. No. 359506)
Structure: Single pass type I membrane protein, extracellular WSXWS-containing cytokine-binding domain, intracellular Box1 and Box2 domains
Distribution: B cells, various levels on NK cells, T cells, NKT cells, B cells, DCs, macrophages, non-hematopoietic cells, including keratinocytes and fibroblasts
Ligand/Receptor: IL-21
Cell Type: B cells, Dendritic cells, Fibroblasts, Macrophages, NK cells, NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856. 2. de Totero D, et al. 2006. Blood 107:3708. 3. Asao H, et al. 2001. J. Immunol. 167:1. 4. Davis ID, et al. 2007. Clin. Cancer Res. 13:6926. 5. Nurieva R. 2007. Nature. 448:480. 6. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144. 7. Ozaki, K, et al. 2000. Proc. Natl. Acad. Sci. USA 97:11439. 8. Parrish-Novak J, et al. 2000. Nature 408:57. 9. Spolski R and Leonard WJ. et al. 2008. Annu. Rev. Immunol. 26:57.
Gene ID: 50615
UniProt: View information about CD360 on UniProt.org
Clone: 17A12
Regulatory Status: RUO
Other Names: IL-21 Receptor, novel interleukin receptor (NILR), MGC10967
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.