Biolegend, 359706, Alexa Fluor® 647 anti-human HIF1α Antibody, 100tests

HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. HIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α. Following nuclear translocation, HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE); only the HIF1 heterodimer can bind DNA. HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in angiogenesis and energy metabolism.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant HIF1α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed Koers J, et al. 2023. Front Immunol. 13:1082154. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed
RRID: AB_2563331 (BioLegend Cat. No. 359705) AB_2563349 (BioLegend Cat. No. 359706)
Structure: 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains
Distribution: Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation
Function: Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations
Interaction: Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2
Biology Area: Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. 2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513. 3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230. 4. Galbraith MD, et al. 2013. Cell. 153:1327.
Gene ID: 3091
UniProt: View information about HIF1alpha on UniProt.org
Clone: 546-16
Regulatory Status: RUO
Other Names: MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha
Isotype: Mouse IgG2b, κ