Biolegend, 360615, Ultra-LEAF™ Purified anti-human CD198 (CCR8) Antibody, 1mg

CCR8, also known as ChemR1, TER1, and CKR-L1, belongs to the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. CCR8 is expressed on some subsets of T cells, monocytes, macrophages, eosinophils, thymocytes, Langerhans cells, neurons, and vascular smooth muscle cells. Binding with its ligand CCL1, CCR8 plays a role in the regulation of monocyte chemotaxis and thymic cell apoptosis. It has been reported that CCR8 is also a receptor for CCL18. CCR8 is also believed to be an alternative coreceptor to CD4 for HIV-1 infection.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCR8-transfectants
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3699003 (BioLegend Cat. No. 360614) AB_3699003 (BioLegend Cat. No. 360615)
Structure: Seven hydrophobic transmembrane domains
Distribution: Vascular smooth muscle cells, macrophages, eosinophils, peritoneal macrophages, thymocytes, T cells, Langerhans cells and neurons
Function: Regulation of monocyte chemotaxis and thymic cell apoptosis
Ligand/Receptor: CCL1, CCL18
Cell Type: Dendritic cells, Eosinophils, Langerhans cells, Macrophages, Neurons, T cells, Thymocytes
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Tiffany HL, et al. 1997. J. Exp. Med. 186:165. 2. Goya I, et al. 1998. J. Immunol. 160:1975. 3. Lee S, et al. 2000. J. Virol. 74:6946. 4. Chensue SW, et al. 2001. J. Exp. Med. 193:573. 5. Qu C, et al. 2004. J. Exp. Med. 200:1231. 6. Isolam SA, et al. 2003. J. Exp. Med. 210:1889.
Gene ID: 1237
UniProt: View information about CD198 on UniProt.org
Clone: L263G8
Regulatory Status: RUO
Other Names: CC-CKR-8,C-C CKR-8, CCR-8, CC chemokine receptor 8, CDw198, CDw198 antigen, CD198, chemokine (C-C motif) receptor 8, chemokine (C-C) receptor 8, TER1, ChemR1, and CKR-L1
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.