Biolegend, 362551, Brilliant Violet 421™ anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Walsh FS, et al. 1981. Nature 289:60. (FC) Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC) Pavlath GK, et al. 1989. Nature 337:570. (FC) Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations: Lellahi SM, et al. 2023. Front Immunol. 13:1058963. PubMed Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed Estep BK, et al. 2022. iScience. 25:105544. PubMed Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed Heger L, et al. 2018. Front Immunol. 9:744. PubMed Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed Grant R, et al. 2021. Methods Protoc. 4:. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
RRID: AB_2566060 (BioLegend Cat. No. 362551) AB_2566061 (BioLegend Cat. No. 362552)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: 5.1H11
Regulatory Status: RUO
Other Names: Leu-19, NKH1, NCAM-1
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.