Biolegend, 362906, Alexa Fluor® 488 anti-human CD191 (CCR1) Antibody, 100tests

CD191, also known as CCR1, is a 41 kD, G-protein coupled receptor expressed predominantly by monocytes. CCR1 is also expressed by a subset of T cells and eosinophils. CCR1 positive cells can migrate in response to a CCL3 and CCL5 gradient. CCR1 knock-out studies suggest that this molecule plays an important role in inflammation and susceptibility to viruses and parasites.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCR1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Parrot T, et al. 2021. JCI Insight. 6:e140074. PubMed
RRID: AB_2563916 (BioLegend Cat. No. 362905) AB_2563917 (BioLegend Cat. No. 362906)
Structure: G-protein coupled receptor, seven transmembrane protein, 41 kD
Distribution: Monocytes, macrophages, subset of T cells, eosinophils
Function: Regulates migration of cells in response to CCL3 and CCL5
Ligand/Receptor: CCL3 and CCL5
Cell Type: Eosinophils, Macrophages, Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Su SB, et al. 1996. J. Leuko. Biol. 60:658. 2. Su S, et al. 1997. Blood. 90:605. 3. Ayehunie S, et al. 1997. Blood. 90:1379. 4. Gerard C, et al. 1997. J. Clin. Invest. 100:2022. 5. Tiffany HL, et al. 1998. J. Immunol. 160:1385. 6. Gilliland CT, et al. 2013. J. Biol. Chem. 288:32194. 7. Bednar F, et al. 2014. J. Immunol. 192:5305.
Gene ID: 1230
UniProt: View information about CD191 on UniProt.org
Clone: 5F10B29
Regulatory Status: RUO
Other Names: CKR1, CD191, HM145, SCYAR1, CMKRB1
Isotype: Mouse IgG1, κ