Biolegend, 366201, Purified anti-PTEN Antibody, 25μg

PTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PTEN protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed western blot, IHC, and ICC testing during product development. We therefore do not recommend this product for any of these applications.
RRID: AB_2922580 (BioLegend Cat. No. 366201)
Structure: PTEN is a 403 amino acid protein with a predicted molecular weight of 47 kD.
Distribution: Ubiquitous/ Nucleus (monoubiquitinated form), Cytoplasm (nonubiquitinated form)
Function: Hydrolase, Protein Phosphatase
Interaction: Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.
Ligand/Receptor: Lipid-binding
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Carracedo A, et al. 2011. Cancer Res. 71:629-33. Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19-65. Mao JH, et al. 2008. Science 321:1499-502. Weng LP, et al. 2001. Hum. Mol. Genet. 10:599-604. Wrighton KH. 2011. Nat Rev Cancer. 11:154. Wang X, et al. 2007. Cell 128:129-39.
Gene ID: 5728
UniProt: View information about PTEN on UniProt.org
Clone: W20041H
Regulatory Status: RUO
Other Names: BZS, TEP1, PTEN1, PTENbeta, Phosphatase and tensin homolog, MMAC1, CWS1, DEC, GLM2, MHAM
Isotype: Rat IgG2b, κ