Biolegend, 366315, PerCP/Cyanine5.5 anti-human CD18 Antibody, 25tests

CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. CD18 non-covalently associates with CD11a, CD11b, or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human LFA-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-11,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Petruzzelli L, et al. 1995. J. Immunol. 155:854. (Activ) Lu C, et al. 2001. J. Immunol. 166:5629. (Activ)
RRID: AB_2728360 (BioLegend Cat. No. 366315) AB_2728361 (BioLegend Cat. No. 366316)
Structure: 90 - 95 kD type I transmembrane glycoprotein.
Distribution: Leukocytes.
Function: Cell adhesion and signaling.
Interaction: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c
Ligand/Receptor: ICAMs.
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer TA. 1994. Cell 76:301.
Gene ID: 3689
UniProt: View information about CD18 on UniProt.org
Clone: CBR LFA-1/2
Regulatory Status: RUO
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.