Biolegend, 367129, Alexa Fluor® 488 anti-human CD14 Antibody, 25tests

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human peripheral blood monocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028. Devitt A, et al. 1998. Nature 6675:505.
Product Citations: Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed Drummer DJ, et al. 2022. Int J Exerc Sci. 15:686. PubMed Halder LD, et al. 2022. MBio. :e0356321. PubMed Halder LD, et al. 2020. Nat Commun. 2.077083333. PubMed
RRID: AB_2721359 (BioLegend Cat. No. 367129) AB_2721360 (BioLegend Cat. No. 367130)
Structure: GPI-linked membrane glycoprotein.
Distribution: Monocytes, macrophages, dendritic cells, and granulocytes.
Function: LPS receptor, clearance of Gram-negative pathogens.
Interaction: LPS.
Ligand/Receptor: LPS.
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Stocks SC, et al. 1990. Biochem. J. 268:275. Wright SD, et al. 1990. Science 4975:1431.
Gene ID: 929
UniProt: View information about CD14 on UniProt.org
Clone: 63D3
Regulatory Status: RUO
Other Names: Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype: Mouse IgG1, κ