Biolegend, 367604, Brilliant Violet 421™ anti-human MERTK Antibody, 100tests

MERTK plays a role in the retinal pigment epithelium as a regulator of rod outer segments fragments phagocytosis. MERTK also plays a role in the inhibition of Toll-like receptor-mediated innate immune responses through the activation of STAT1. Upregulation of MERTK seems to also promote the survival of certain cancer cells, such as t(1;19)-positive acute lymphoblastic leukemias (ALL). MERTK also has a role in cellular migration, as MERTK KO macrophages demonstrate cytoskeletal disruptions that impacts its shape and directional migration. Melanoma cells express high levels of MERTK, which makes this molecule an attractive therapeutic target.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MERTK extracellular domain/Fc fusion.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Rogers AE, et al. 2012. Oncogene 31:4171.
Product Citations: Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed Zheng X, et al. 2020. Cancer Res. 4414:80. PubMed Holtzhausen A, et al. 2019. Cancer Immunol Res. 7:1672. PubMed
RRID: AB_2566396 (BioLegend Cat. No. 367603) AB_2566397 (BioLegend Cat. No. 367604)
Structure: Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.
Distribution: Macrophages, monocytes, and dendritic cells.
Function: TLR regulation, efferocytosis, phagocytosis regulation, cell survival, and macrophage migration.
Interaction: GRB2, PLCG2, and TNK2.
Ligand/Receptor: LGALS3, TUB, TULP1, and GAS6.
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Protein Kinases/Phosphatase, Toll Like Receptors
Antigen References: 1. Schlegel J, et al. 2013. J. Clin. Invest. 123:2257. 2. Chen J, et al. 1997. Oncogene 14:2033. 3. Yefimova MG, et al. 2013. Autophagy 9:653. 4. Zhang W, et al. 2013. J. Med. Chem. 56:9693. 5. Lee YJ, et al. 2012. J. Leuko. Biol. 91:921. 6. Krause S, et al. 2015. Blood 125:820. 7. Tang Y, et al. 2015. PLoS One 10:e0117787.
Gene ID: 10461
UniProt: View information about MERTK on UniProt.org
Clone: 590H11G1E3
Regulatory Status: RUO
Other Names: C-Mer Proto-Oncogene Tyrosine Kinase, c-mer, Proto-Oncogene C-Mer, MER Receptor Tyrosine Kinase, STK Kinase, Receptor Tyrosine Kinase MerTK, Tyrosine-Protein Kinase Mer, MER, RP38, c-Eyk, Tyro12
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.