Biolegend, 368404, Alexa Fluor® 647 anti-human Ikaros Antibody, 100tests

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
RRID: AB_2566182 (BioLegend Cat. No. 368403) AB_2566183 (BioLegend Cat. No. 368404)
Structure: Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution: Fetal and adult hematopoietic cells.
Function: Regulates lymphocyte differentiation.
Interaction: DNA.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930. 2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736. 3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.
Gene ID: 10320
UniProt: View information about Ikaros on UniProt.org
Clone: 16B5C71
Regulatory Status: RUO
Other Names: IK1, IKZF1
Isotype: Mouse IgG2a, κ