Biolegend, 368514, Alexa Fluor® 700 anti-human CD45 Antibody, 100tests

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Application References(PubMed link indicates BioLegend citation): Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations: Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed Bonavia AS, et al. 2022. Front Immunol. 13:1007016. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Zheng X, et al. 2020. Cancer Res. 4414:80. PubMed Wang J, et al. 2020. Cell. 183:1264. PubMed Perry JSA, et al. 2018. Immunity. 48:923. PubMed Solimani F, et al. 2019. Front Immunol. 1.672222222. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed Fabián-Morales E, et al. 2021. Front Psychiatry. 12:753562. PubMed Yankova E, et al. 2021. Nature. 593:597. PubMed Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed Pastar I, et al. 2021. J Clin Invest. :e133727. PubMed LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed Moreno-Fernandez ME, et al. 2021. STAR Protoc. 2:100937. PubMed Shen XR, et al. 2021. Viruses. 13:. PubMed Roerden M, et al. 2021. Cancers. 13(11):. PubMed Morton B, et al. 2021. Nat Commun. 12:3554. PubMed Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
RRID: AB_2566373 (BioLegend Cat. No. 368513) AB_2566374 (BioLegend Cat. No. 368514)
Structure: Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets.
Function: Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor: Galectin-1, CD2, CD3, and CD4.
Cell Type: B cells, Dendritic cells, Neutrophils
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: 2D1
Regulatory Status: RUO
Other Names: Leukocyte Common Antigen (LCA), T200
Isotype: Mouse IgG1, κ