Biolegend, 369005, PE anti-human uPA (PLAU) Antibody, 25tests

uPA is a serine protease. It is produced as a single-chain protein containing serine protease domain, Kringle domain, and growth factor domain. It is proteolyticaly cleaved between Lys158 and Ile159; the two resulting chains are kept together as a dimer by a disulfide bond. The primary function of uPA is to convert plasminogen into plasmin by specific cleavage of an Arg-Val bond in plasminogen. uPA is also involved in the degradation of the extracellular matrix and possibly tumor cell migration and proliferation. Alternatively spliced transcript variants encoding different isoforms have been found for uPA; a specific polymorphism of uPA may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified urokinase
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Heidtmann H, et al. 1989. Cancer Research 49:6960. Schwartz-Albiez R, et al. 1992. Br. J. Cancer 65:51.
RRID: AB_2571974 (BioLegend Cat. No. 369005)
Structure: 411-aa residue zymogen that is cleaved into two chains and forms heterodimer.
Distribution: Urine, blood, and extracellular matrix.
Function: Serine protease.
Ligand/Receptor: u-PA Receptor, CD87.
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: 1. Riccio A, et al. 1985. Nucleic Acids Res. 13:2759.
Gene ID: 5328
UniProt: View information about uPA on UniProt.org
Clone: UK98/6
Regulatory Status: RUO
Other Names: Urokinase-type Plasminogen activator, urokinase, Plasminogen activator, ATF, BDPLT5, QPD, UPA, URK
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.