Biolegend, 369705, APC anti-human Arginase I Antibody, 25tests

Arginase I, also known as ARG1, is a 34.7kD protein expressed by neutrophils and myeloid-derived suppressor cells (MDSCs). There are two isoforms that are differentiated based on their tissue distribution and subcellular localization. Arginase I converts L-arginine into L-ornithine and urea; it is the final enzyme in the urea cycle. While mostly found in the liver, it can also be expressed in cells lacking a comprehensive urea cycle. Also, it contributes to vasodilation and vascular function. Arginase I is also reported to be involved in MDSC mediated suppression of T cell proliferation.  Arginase I has been implicated in hyperargininemia (decreased function of arginase I) and Q fever.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant protein expressed in E.coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Wang HY, et al. 2023. Exp Hematol Oncol. 12:35. PubMed
RRID: AB_2814328 (BioLegend Cat. No. 369705) AB_2814329 (BioLegend Cat. No. 369706)
Structure: Belongs to the ureohydrolase family and has a molecular mass of 34.7 kD.
Distribution: Neutrophils and myeloid-derived suppressor cells (MDSC).
Function: Converts L-arginine into L-ornithine and urea.
Interaction: Arginine.
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: 1. Munder M, et al. 2005. Blood 105:2549.2. Luckner-Minden C, et al. 2010. JLB. 87:1125.3. Holowatz L, et al. 2006. Journal of Physiology 574:573.4. Sin YY, et al. 2013. PLoS One 8:11.5. Benoit M, et al. 2008. Eur. J. Immunol. 4:1065.
Gene ID: 383
UniProt: View information about Arginase I on UniProt.org
Clone: 14D2C43
Regulatory Status: RUO
Other Names: ARG1, Liver Arginase, Type 1 Arginase, Arginase-1, Liver-Type Arginase
Isotype: Mouse IgG2b, κ