Biolegend, 371011, APC anti-human Aiolos Antibody, 25tests

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2910418 (BioLegend Cat. No. 371011) AB_2910418 (BioLegend Cat. No. 371012)
Structure: 58kD, zinc finger protein
Distribution: T and B lymphocytes
Function: Hematopoetic-specific transcriptional activator and chromatin remodeler
Interaction: Forms heterodimers with Ikaros family members IKZF4 and IKZF5
Cell Type: B cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Zhuang Y, et al. 2015. Oncol Rep. 33:1319-25. Ma S, et al. 2008. Blood. 111:1396-403. Billot K, et al. 2010. Leuk Res. 34:289-93. Morgan B, et al. 2004. EMBO J. 16:2004. Wang JH, et al. 1998. Immunity. 9:543-53. Hosokawa Y, et al. 1999. Genomics. 61:326-9.
Gene ID: 22806
UniProt: View information about Aiolos on UniProt.org
Clone: 14C4C97
Regulatory Status: RUO
Other Names: IKZF3, AIO, ZNFN/A3
Isotype: Mouse IgG1, κ