Biolegend, 371306, PE anti-human CD99 Antibody, 100tests

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human thymus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Waclavicek M, et al. 1998. J. Immunol. 161:4671. Pickl W, et al. 2001. J. Virol. 75:7175.
Product Citations: Yeremenko N, et al. 2013. Arthritis Rheum. 174:65. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
RRID: AB_2616973 (BioLegend Cat. No. 371305) AB_2616974 (BioLegend Cat. No. 371306)
Structure: Surface glycoprotein, type I transmembrane protein, 32kD.
Distribution: T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.
Function: Leukocyte migration, T-cell adhesion, T-cell death- caspase independent, and spontaneous rosette formation with erythrocytes.
Interaction: Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
Cell Type: Leukemia, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Gelin C, et al. 1989. EMBO. 8:3253. 2. Goodfellow PJ, et al. 1986. Science 234:740. 3. Pettersen RD, et al. 2001. J. Immunol. 166:4931.
Gene ID: 4267
UniProt: View information about CD99 on UniProt.org
Clone: 3B2/TA8
Regulatory Status: RUO
Other Names: CD99, MIC2, HBA71, MSK5X, E2 antigen
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.