Biolegend, 371404, PE anti-human CD92 Antibody, 100tests

CD92, also known as CDW92 and CTL1, is a 70kD choline transporter-like transmembrane protein. CD92 is important for transport of choline across the membrane for synthesis of cell membrane components and the neurotransmitter, acetylcholine. CD92 is primarily expressed on monocytes and neutrophils, but can also be found on other myeloid and T-cell subsets. Ionomycin or calcium ionophore mediates the maturation of monocytic dendritic cells causing down-regulation of CD92 but treatment with IL-10 causes re-expression.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MV4-11
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Wille S, et al. 2001. J. Immunol. 167:5795.
RRID: AB_2616926 (BioLegend Cat. No. 371403) AB_2616927 (BioLegend Cat. No. 371404)
Structure: 70kD choline transporter-like transmembrane protein.
Distribution: Monocytes, neutrophils, and certain groups of T-cells.
Function: Transport of choline through the cell membrane.
Interaction: Choline.
Cell Type: Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schenkel LC, et al. 2015. FASEB J. 29:1663. 2. Yamada T, et al. 2011. Neurochem. Int. 58:354. 3. Fullerton MD, et al. 2006. Am. J. Physiol. Cell Physiol. 290:C1230.
Gene ID: 23466
UniProt: View information about CD92 on UniProt.org
Clone: VIM15b
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CD92, CDw92, CTL1, SLC44A1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.