Biolegend, 371806, APC anti-human STAT3 Antibody, 100tests

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 15H2B45 blocks clone 4G4B45.
RRID: AB_2927833 (BioLegend Cat. No. 371805) AB_2927833 (BioLegend Cat. No. 371806)
Structure: 770 amino acids with a predicted molecular weight of 88.1 kD. Contains a C-terminal SH2 domain responsible for homo- or heterodimerization.
Distribution: Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to nucleus to act as transcriptional activator. Activates a wide variety of genes involved in cell growth and apoptosis, involved in gp130-related signaling.
Function: Cytoplasm.
Interaction: STAT3 forms a homodimer or a heterodimer with other STAT family members. Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lufei C, et al. 2003. EMBO J. 22:1325. 2. Deo D, et al. 2002. J. Biol. Chem. 277:21237. 3. Pfeffer L, et al. 1997. Science. 276:1418. 4. Akira S, et al. 1994. Cell. 77:63.
Gene ID: 6774
UniProt: View information about STAT3 on UniProt.org
Clone: 15H2B45
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, acute-phase response factor (APRF), HIES, ADMIO
Isotype: Mouse IgG1, κ