Biolegend, 372207, PE anti-human/mouse Granzyme B Recombinant Antibody, 25tests

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Wang X, et al. 2023. Nat Commun. 14:3440. PubMed Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed Wang W, et al. 2022. Cell Rep. 41:111582. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Li J, et al. 2022. Front Immunol. 13:1043484. PubMed Lin J, et al. 2023. Nat Commun. 14:224. PubMed Jiang X, et al. 2023. Cell Discov. 9:7. PubMed Chen M, et al. 2023. Cell Biosci. 13:19. PubMed Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed Allen SD, et al. 2021. Biomaterials. 269:120635. PubMed Yang Z, et al. 2022. J Immunother Cancer. 10:. PubMed Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed Liu X, et al. 2020. Nature. . PubMed Yang X, et al. 2021. Front Pharmacol. 12:771046. PubMed Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Alsaleh G, et al. 2020. Elife. 9: . PubMed Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed Hubrack S, et al. 2021. Sci Rep. 11:14090. PubMed Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed Feng Y, et al. 2022. Life (Basel). 12:. PubMed Kim S, et al. 2020. Autophagy. :1. PubMed Li Z, et al. 2020. J Immunol. 2589:204. PubMed Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed Zheng Y, et al. 2022. J Immunol. 208:501. PubMed Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed Li ZY, et al. 2021. J Exp Med. 218:. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed Khurana P, et al. 2021. Front Immunol. 12:700374. PubMed Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
RRID: AB_2687031 (BioLegend Cat. No. 372207) AB_2687032 (BioLegend Cat. No. 372208)
Structure: 32 kD serine protease
Distribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction: Caspase-3
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: T cells, NK cells, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Proteases, Enzymes and Regulators
Antigen References: 1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 300214939
UniProt: View information about Granzyme B on UniProt.org
Clone: QA16A02
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.