Biolegend, 373410, PE/Cyanine7 anti-human CD18 Antibody, 100tests

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral blood lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes.
Application References(PubMed link indicates BioLegend citation): Singer II, et al. 1993. J. Immunol. 150:2844.Seth R, et al. 1991. FEBS Lett. 282:193.
Product Citations: Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
RRID: AB_2716025 (BioLegend Cat. No. 373409) AB_2716026 (BioLegend Cat. No. 373410)
Structure: Integrin, type I transmembrane glycoprotein
Distribution: Leukocytes
Function: Adhesion and signaling in hematopoietic cells
Interaction: Associates with CD11a, CD11b, or CD11c
Ligand/Receptor: ICAMs
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hogg N, et al. 1999. J. Clin. Invest. 103:97. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Springer TA. 1994. Cell 76:301.
Gene ID: 3689
UniProt: View information about CD18 on UniProt.org
Clone: 1B4/CD18
Regulatory Status: RUO
Other Names: Integrin beta-2, integrin beta chain, beta 2
Isotype: Mouse IgG2a, κ