Biolegend, 374206, PE anti-human CD86 Antibody, 100tests

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ARH 77 (B lymphoblastoid cell line).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Rodriguez-Perdigon M, et al. 2023. Front Bioeng Biotechnol. 11:1159819. PubMed Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed Su R, et al. 2020. Cancer Cell. 38(1):79-96.e11. PubMed Eken A, et al. 2020. North Clin Istanb. 0.513194444. PubMed Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed Rodriguez-Perdigon M, et al. 2022. Macromol Biosci. 22:e2200168. PubMed Wang J, et al. 2022. Oncoimmunology. 11:2016159. PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed Zhang B, et al. 2020. Cell Death Dis. 0.973611111. PubMed Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed Li F, et al. 2022. iScience. 25:105147. PubMed
RRID: AB_2721632 (BioLegend Cat. No. 374205) AB_2721633 (BioLegend Cat. No. 374206)
Structure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution: Monocytes/macrophages, activated B cells and T cells, dendritic cells
Function: T Cell activation
Interaction: C-Jun, NFκB
Ligand/Receptor: CD28, CD152
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131. 2. June C, et al. 1994. Immunol. Today 15:321.
Gene ID: 942
UniProt: View information about CD86 on UniProt.org
Clone: BU63
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B7-2, B70, Ly-58
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.