Biolegend, 376915, Brilliant Violet 605™ anti-human TLR7 Antibody, 25tests

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.
RRID: AB_3717099 (BioLegend Cat. No. 376915)
Structure: Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution: Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes
Function: Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Interaction: B cells, Dendritic cells
Ligand/Receptor: Single-stranded RNA
Cell Type: B cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling
Gene ID: 51284
UniProt: View information about TLR7 on UniProt.org
Clone: S18024F
Regulatory Status: RUO
Other Names: Toll-like receptor (TLR) 7, CD287
Isotype: Rat IgG2a, κ