Biolegend, 377122, PE anti-mouse/human TOX Antibody, 25μg

TOX, a member of the high-mobility group (HMG) box superfamily of DNA-binding proteins, was originally identified as a thymic transcript expressed in CD4 + CD8 + double-positive T cells. Along with TOX2, TOX3, and TOX4, TOX is also part of a small subfamily of transcription factors that share conserved HMG-box sequences. TOX is highly upregulated in thymocytes undergoing positive selection and is required for the differentiation and development of various T cell subsets, including CD4 + T cells, regulatory T cells, and natural killer T cells (NKTs). TOX-deficient mice fail to develop lymph nodes and have significantly diminished numbers of CD4 + T, NK, and ILC cells.
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse TOX recombinant protein (21-236 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Use of True-Nuclear™ Transcription Factor or True-Phos™ Perm Buffer are recommended for TOX detection with clone W17072A Clone W17072A does not block the staining of reference clones TXRX10, REA473 and NAN448B tested.
Structure: 562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.
Distribution: Nucleus
Function: TOX is a transcription factor required for the development of various T lymphocyte subsets.
Interaction: TOX interacts with ZDHHC17
Biology Area: Transcription Factors
Antigen References: Spits H. 2015. Nat Immunol. 16:594. Seehus CR, et al. 2015. Nat Immunol. 16:599. Huang Y, et al. 2015. Blood. 125:1435. Aliahmad P, et al. 2012. Curr Opin Immunol. 24:173. Di Santo JP. 2010. Nat Immunol. 11:885. Aliahmad P, et al. 2004. J Exp Med. 199:1089.
Gene ID: 252838
UniProt: View information about TOX on UniProt.org
Clone: W17072A
Regulatory Status: RUO
Other Names: Thymocyte Selection-Associated High Mobility Group Box, Thymus High Mobility Group
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.