Biolegend, 377129, PE anti-human MRP-14 (S100A9) Antibody, 100tests

The S100A8/A9 complex has been shown to play a role in calcium-dependent activation and exhibits antibacterial, antifungal, immunomodulatory, and anti-proliferative effects. It also functions as a chemoattractant for neutrophils and serves as an endogenous activator of Toll-like receptor 4 (TLR4). S100A9 has been identified as a marker of CD14⁺ HLA-DR⁻/low myeloid-derived suppressor cells (MDSCs).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein of human MRP-14 (S100A9)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3720634 (BioLegend Cat. No. 377128) AB_3720634 (BioLegend Cat. No. 377129)
Structure: S100A9 belongs to S100 family of proteins containing 2-EF-hand calcium-binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8.
Distribution: Secreted and localized to the cytoplasm and cell membrane in cells such as MDSCs, neutrophils, monocyte, and ileal tissue eosinophils
Function: Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Cell Type: Eosinophils, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Adhesion Molecules
Antigen References: Loser K, et al. 2010. Nat. Med. 16:713. Cheng P, et al. 2008. J. Exp. Med. 205:2235. Leukert N, et al.2006. J. Mol. Biol. 359:961. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID: 6280
UniProt: View information about MRP-14 on UniProt.org
Clone: A22135F
Regulatory Status: RUO
Other Names: S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.