Biolegend, 378704, PE anti-human CD238/KEL Recombinant Antibody, 100tests

QA20A42 recognizes human CD238 also known as the kell blood group antigen Kpbc, KELL and ECE3. CD238 is an approximately 115 kD type II transmembrane glycoprotein that is encoded by the KEL gene. CD238 is expressed on erythrocytes, human embryonic stem cell-derived endoderm and on cells from various other tissues, including testis, heart, brain and skeletal muscle. CD238 functions as a zinc endopeptidase that cleaves endothelin-3 into an active form which can function as a vasoconstrictor. Decreased expression of CD238 is associated with the McLeod phenotype as a consequence of losing XK protein expression.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone QA20A42 can completely block the binding of positive clone to target cells.
RRID: AB_2936750 (BioLegend Cat. No. 378703) AB_2936750 (BioLegend Cat. No. 378704)
Structure: Type II transmembrane glycoprotein 93-115 kD
Distribution: Erythrocytes, human embryonic stem cell-derived endoderm and on cells from various other tissues
Function: Zinc endopeptidase that cleaves endothelin-3 into an active form which can function as a vasoconstrictor. Blood transfusions and also specific autoimmune diseases related to Kell antigen recognition.
Ligand/Receptor: Laminin α5
Cell Type: Embryonic Stem Cells, Erythrocytes
Biology Area: Immunology
Molecular Family: Blood Group Markers, CD Molecules
Antigen References: Daniels G. 2009. Hum Genet. 126:729-42. Denomme GA. 2015. Immunohematology. 31:14-9.
Gene ID: 3792
UniProt: View information about CD238/KEL on UniProt.org
Clone: QA20A42
Regulatory Status: RUO
Other Names: Kell blood group glycoprotein, Kell blood group antigen Kpbc, KELL and ECE3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.