Biolegend, 379403, PE anti-human IL-1RL1 (ST2) Antibody, 25tests

IL1RL1 is a member of the Toll-like receptor superfamily the receptor for Interleukin-33. It is a 556 amino acid single-pass type I membrane protein. ST2 exists in two forms as splice variants: a soluble form, which acts as a decoy receptor and sequesters free IL-33, and a membrane-bound form (ST2), which activates the MyD88/NF-κB signaling pathway. Signaling through IL1RL1 drives cytokine production in mast cells, basophils, eosinophils, invariant NKT and NK cells, Th2 lymphocytes, and type 2 innate immune cells. IL-33 signaling is required for maintaining the suppressive function of regulatory T cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-1RL1, AA19-328
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2922608 (BioLegend Cat. No. 379403) AB_2922608 (BioLegend Cat. No. 379404)
Structure: Single-pass type I membrane protein
Distribution: Th2 lymphocytes, mast cells, eosinophils, basophils, smooth muscle cells, endothelial cells
Function: Cytokine signaling
Interaction: Signaling requires association of the coreceptor IL1RAP. Recruits MYD88, IRAK1, IRAK4, and TRAF6.
Ligand/Receptor: IL-33
Cell Type: Basophils, Endothelial cells, Eosinophils, Lymphocytes, NK cells, Th2, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Schmitz J, et al. 2005. Immunity. 23: 479-490. Brint E, et al. 2004. Nat Immun. 5:373-379. Griesenauer B, et al. 2017. Front Immunol. 8:475.
Gene ID: 9173
UniProt: View information about IL-1RL1 on UniProt.org
Clone: A16008J
Regulatory Status: RUO
Other Names: IL-33R, ST2, IL-1RL1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.